goat anti-darpp32 Search Results


goat anti-darpp32  (Absolute Biotech Inc)
90
Absolute Biotech Inc goat anti-darpp32
a , Western blots of the indicated brain regions from 8-week-old R6/2 mice and WT littermate controls. HDGF band is indicated on the right, the upper band is unspecific. Total protein detection was used as loading control. b , Quantification of HDGF protein quantity in the indicated brain regions of 8- and 12-week-old R6/2 mice, normalized first to total protein and then to the average value of the littermate controls. N=4-5 mice per group. Unpaired two-tailed t-test, per brain region and age group. No significant differences were observed. CE, cerebellum; HP, hippocampus; CX, cortex; ST, striatum. c , Images of brain sections from 12-week-old R6/2 and WT mice immunostained for HDGF. <t>DARPP32</t> and neurogranin (NGRN) were used as markers of striatal MSNs and cortical PCs, respectively. Nuclei were counterstained with DAPI. d , Quantification of HDGF immunofluorescence intensity in striatal MSNs and cortical PCs of R6/2 mice and WT controls. Values were background-subtracted and normalized to the fluorescence intensity of the highest expressing cell in the field of view. N=3 mice per group. Unpaired two-tailed t-test, per cell type. No significant differences were observed. Scale bar in c, 20 µm.
Goat Anti Darpp32, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti-darpp32/product/Absolute Biotech Inc
Average 90 stars, based on 1 article reviews
goat anti-darpp32 - by Bioz Stars, 2026-03
90/100 stars
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a , Western blots of the indicated brain regions from 8-week-old R6/2 mice and WT littermate controls. HDGF band is indicated on the right, the upper band is unspecific. Total protein detection was used as loading control. b , Quantification of HDGF protein quantity in the indicated brain regions of 8- and 12-week-old R6/2 mice, normalized first to total protein and then to the average value of the littermate controls. N=4-5 mice per group. Unpaired two-tailed t-test, per brain region and age group. No significant differences were observed. CE, cerebellum; HP, hippocampus; CX, cortex; ST, striatum. c , Images of brain sections from 12-week-old R6/2 and WT mice immunostained for HDGF. DARPP32 and neurogranin (NGRN) were used as markers of striatal MSNs and cortical PCs, respectively. Nuclei were counterstained with DAPI. d , Quantification of HDGF immunofluorescence intensity in striatal MSNs and cortical PCs of R6/2 mice and WT controls. Values were background-subtracted and normalized to the fluorescence intensity of the highest expressing cell in the field of view. N=3 mice per group. Unpaired two-tailed t-test, per cell type. No significant differences were observed. Scale bar in c, 20 µm.

Journal: bioRxiv

Article Title: Neuroprotective effects of hepatoma-derived growth factor in models of Huntington’s disease

doi: 10.1101/2023.02.23.529222

Figure Lengend Snippet: a , Western blots of the indicated brain regions from 8-week-old R6/2 mice and WT littermate controls. HDGF band is indicated on the right, the upper band is unspecific. Total protein detection was used as loading control. b , Quantification of HDGF protein quantity in the indicated brain regions of 8- and 12-week-old R6/2 mice, normalized first to total protein and then to the average value of the littermate controls. N=4-5 mice per group. Unpaired two-tailed t-test, per brain region and age group. No significant differences were observed. CE, cerebellum; HP, hippocampus; CX, cortex; ST, striatum. c , Images of brain sections from 12-week-old R6/2 and WT mice immunostained for HDGF. DARPP32 and neurogranin (NGRN) were used as markers of striatal MSNs and cortical PCs, respectively. Nuclei were counterstained with DAPI. d , Quantification of HDGF immunofluorescence intensity in striatal MSNs and cortical PCs of R6/2 mice and WT controls. Values were background-subtracted and normalized to the fluorescence intensity of the highest expressing cell in the field of view. N=3 mice per group. Unpaired two-tailed t-test, per cell type. No significant differences were observed. Scale bar in c, 20 µm.

Article Snippet: The following primary antibodies were used: rabbit anti-HDGF (Abcam, ab128921, 1:500), mouse anti-HTT (EM48, Chemicon, MAB5374, 1:500), mouse anti-Flag (Origene, TA50011, 1:1,000), goat anti-ChAT (Chemicon, AB144, 1:500), goat anti-DARPP32 (LifeSpan Biosciences, LS-C150127, 1:300), mouse anti-NGRN (R&D Systems, MAB7947, 1:60), chicken anti-GFAP (Origene, AP31806PU-N, 1:2,000, with antigen retrieval), mouse anti-APC (Calbiochem, OP80, 1:20, with antigen retrieval), and goat anti-IBA1 (Abcam, ab107159, 1:1,000, with antigen retrieval).

Techniques: Western Blot, Two Tailed Test, Immunofluorescence, Fluorescence, Expressing